Mycoprotein 2.0

As prosperity rises, demand for meat increases as it is a rich source of protein. This in turn places demand on water resources, changes land use (in a manner highly dependent upon how the animal is fed) and leads to an increase in anthropogenic GHG emissions. This has been determined to be unsustainable by a number of international bodies, with some estimates predicting a 70% rise from current levels of 11% of total GHG emissions by 2050. However, demand for protein can also be met by crop-based sources (e.g. soy and pulses) and by mycoprotein, produced by fermentation of crop-derived glucose into biomass, which is harvested and processed into high quality protein.

Mycoprotein remains a relatively under-exploited resource worldwide but offers great promise for year-round production of high quality protein, a vital requirement for future food security and human nutrition. The most significant challenge to production is the reliance on a single carbon source, a wheat-derived glucose, which requires special processing before it is suitable for use. Our recent work has revealed that while the fungus used to produce mycoprotein is grown on this glucose substrate, production of a number of essential vitamins is inhibited. Our recent work has revealed that expression of vitamins in some other carbon sources, for example beet derived sucrose syrup is observed. In some, but not all cases, this is coupled to an increase in other deleterious secondary metabolites. This leads to the question, how is the fungus regulating secondary metabolism in relation to carbon source?

To expand both the nutritional value of mycoprotein and the range of carbon sources that can be utilised (enabling production to move to other regions of the world) we will use the latest DNA sequencing techniques to reveal the structure of the genome of Fusarium venenatum and study the regions of the genome that contain secondary metabolite genes. From work carried out in other related fungi it is knownthat control of secondary metabolism (SM) is regulated by the position of SM cluster in the genome, and by specific regulatory factors. Utilising the latest sequencing techniques will allow us to positionally resolve SM location and determine the underlying mechanisms regulating responses to different carbon sources. Through a series of controlled batch and continuous culture experiments we will develop techniques to selectively induce vitamin biosynthesis across a range of carbon sources, without inducing the expression of deleterious SM genes, providing both an understanding of the control of SM and an enhanced product for future product development. Building on our existing work we will expand the toolbox of molecular techniques in order to edit the genome of F. venenatum to remove deleterious secondary metabolite gene clusters and their regulatory factors which are induced in response to different carbon sources. As a result of this work, mycoprotein will be able to be produced using a larger range of carbon sources drawing upon a wider range of UK agricultural sources (maize, barley, rice) and even shift to sucrose-based production of mycoprotein (a carbon source that has currently been completely inaccessible), utilising UK sources of sucrose such as sugar beet. Furthermore, the ability to enhance the complement of micronutrients in mycoprotein will broaden its utility as an important component of global diets and offers a more sustainable and flexible alternative to meat.

Principal Investigator: Dr Richard Harrison

Researchers: Fiona Wilson

Funder: BBSRC

Industry Partner: Marlow Foods

A chemostat, used for continuous production of small levels of mycelial culture for genetic study.

Targeted gene knockout allows functional characterisation of genes involved in growth and metabolism.

Each year, as summer fades to autumn, enthusiastic gardeners often contact East Malling Research (EMR) with samples of apples, keen to know what variety they have growing in their garden. Perhaps they have moved house and ‘inherited’ a new tree (or trees), or maybe lost the label from a tree given as a gift. For many reasons finding out exactly what is growing in the garden can be highly desirable.

EMR doesn’t offer a free variety identification service, but we do have lots of helpful advice for this rather specialist activity.

The single best method of getting your fruit identified is to take a sample of fruit and leaf material to one of the many identification events held each autumn. These are advertised on-line and are held up and down the country – when you are at the event, the fruit variety identification is usually free. The Royal Horticultural Society (RHS) organises a number of these events per year with dates advertised annually on their website.

Brogdale Collections, home of the National Fruit Collection, offer a £20 per variety postal service. You should allow approximately 8 weeks minimum for reply, sometimes longer in the busy peak months of October, November and December. Full information on this great service can be found by clicking here.

As an alternative to taking fruit to an expert, we highly recommend the fantastic ’fruitID’ website which can be found at http://www.fruitid.com/ (or by googling the term ‘fruitID’ or putting ‘fruitID’ in your  internet browser’s search facility). ‘fruitID’ isn’t primarily intended for public use, but the search options, images and resulting background varietal information are now second to none. Well worth a look, even if you have had your variety identified by an expert.

Of course, even expert ID judgement isn’t a 100% guarantee of accuracy and there is one last option. EMR offers a DNA Fingerprinting Service – this may be an expensive option for individual samples for ‘amateur interest’, but it does produce the most reliable answer possible. Moreover, this service utilises leaves or fruit (young leaves are best), so you don’t have to wait until the autumn or send off your valued home-produced fruit! More information on this service can be found here.

The fruitID website is an invaluable reference source for apple varieties. Extension of the site to include additional crops, e.g.pears are being planned.

The DNA Fingerprinting Service, based at EMR, offers accurate identification of several crops including: apples, pears, cherries, strawberries and raspberries.

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